BT8361 Microbiology Laboratory Lab Manual Viva Experiments Set Previous
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BT8361 MICROBIOLOGY LABORATORY
Experiments
1. Introduction, Laboratory Safety, Use of Equipment;
Sterilization Techniques
2. Culture Media-Types and Use; Preparation of Nutrient
broth and agar
3. Culture Techniques, Isolation and Preservation of
Cultures- Broth: flask, test tubes; Solid:Pour plates, streak plates, slants, stabs
4. Microscopy – Working and care of Microscope
5. Microscopic Methods in the Study of Microorganisms.,
Microscopic identification of yeast/mould
6. Staining Techniques Simple, Differential- Gram’s
Staining, spore /capsule staining
7. Quantification of Microbes: Sampling and Serial Dilution;
Bacterial count in Soil – TVC
8. Effect of Disinfectants- Phenol Coefficient
9. Antibiotic Sensitivity Assay
10. Growth Curve in Bacteria and Yeast
11. Effect of pH, Temperature, UV radiation on Growth
Bacteria
TEXT BOOKS
1. Cappuccino, J.G. and N. Sherman “Microbiology: A
Laboratory Manual”, 4th Edition,
Addison-Wesley, 1999.
2. Collee, J.G. etal., “Mackie & McCartney Practical
Medical Microbiology” 4th Edition,
ChurchillLivingstone, 1996.
BT8361 Microbiology Laboratory Lab Manual
BT8361 Microbiology Laboratory Viva Questions
BT8361 Microbiology Laboratory Output
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