BT8311 CELL BIOLOGY LABORATORY Lab Manual Syllabus Viva Question Regulation 2017

BT8311 CELL BIOLOGY LABORATORY Lab Manual Syllabus Viva Experiments  Set  Previous Important Question With Answers  Aim Algorithm Program Procedure  Result  Output  Anna University Regulation 2017 Study Materials Pdf Ppt 

BT8311 CELL BIOLOGY LABORATORY

LIST OF EXPERIMENTS

1. Introduction to principles of sterile techniques and cell propagation

2. Principles of microscopy, phase contrast and fluorescent microscopy

3. Identification of given plant, animal and bacterial cells & their components by microscopy

4. Gram’s Staining

5. Leishman Staining

6. Giemsa Staining

7. Thin Layer Chromatography

8. Separation of Peripheral Blood Mononuclear Cells from blood

9. Osmosis and Tonicity

10. Tryphan Blue Assay

11. Staining for different stages of mitosis in AlliumCepa (Onion)

REFERENCES:

1. Rickwood, D. and J.R. Harris “Cell Biology: Essential Techniques”, Johnwiley, 1996.

2. Davis, J.M. “Basic Cell Culture: A Practical Approach”, IRL, 1994.Equipment Needed for 30 Students Autoclave 1 Hot Air Oven 1Incubators 2 Light Microscopes 4 10 Incubator Shaker 1 Colorimeter 2  Lamina Flow Chamber 2 Glassware, Chemicals, Media as required

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